The New Brunswick Scientific software, BIOCOMMAND LITE, is used for data acquisition and for on-line plots of the various parameters. See the manual for complete details of the software.

1. Open BCLite from the Desktop.
2. In the first window select Open an Existing Recipe.
3. Select File name Recipe Bio Bob.rcp.
4. Go back to desktop
5. Open the shortcut to OptoDisR.
   1. You should see the dO2, Agitation, Temperature, and pH logging.
6. Go back to BioCommand Lite.
7. Click Start Batch.
8. Select no, do not save changes to recipe.
9. Enter a novel Batch Display Name and then a Destination File Name.
10. After you click OK for the Destination File Name data will being recording.
11. To monitor/change set points go to Views, and double click Batch Summary.1. To change a set point, double click on the line for that controlled (e.g. Agit)
12. To monitor trends and history go to Views. Double click on Trend under New Views and click Loops. Then click Set up Loop and select the loop you wish to monitor (e.g. dO2, pH, or so on). Use the arrow keys to add additional loops to the plot.
13. To add an alarm, go to the Alarm Tab, and choose the controller and set alarm bounds.
14. When finished gathering data, click End Batch on the BioCommand software, and click Exit Opto in the OptoDisR software.
15. You can open OptoDisplay results in Excel.
    1. Open Excel.
    2. Go to file open.
    3. My Computer C: Drive.
    4. Open Opto Data folder.
    5. Change Files of Type to All Files.
    6. The logs are saved by date, so find the correct file.
    7. When the text import wizard opens, click Next.
    8. In Step 2, put a check in comma and hit next.
    9. Now select Finish.
    10. When it is open, columns G & H do not pertain to our reactor.
16. You can later read these files using separate software by New Brunswick, DBViewer32, and then transfer the file to MS Excel.
    1. Open the DBViewer32 program.
    2. Select Open and find the file name you gave to your experiential run in Step 9.